PCR Nucleic Acid Testing

Sampling Recommendations for Pet PCR Nucleic Acid Testing

Sample Type  Sampling Method         Pathogen Eye, nose, and throat swab Eye swab: moisten the swab head with sample preservation solution, and then gently wipe under the pet’s eye and conjunctiva 3-5 times; Tears can be appropriately collected as a sample. Nasal swab: collect by gently rubbing the tip of a disposable swab under the pet’s nasal…

Cats have diarrhea but can’t find the cause

Cats have diarrhea but can’t find the cause? You may be ignoring the virus infection!

With the development of society, pets are regarded as members of the family, and their health is increasingly valued by parents. We have made detailed introductions on the digestive tract pathogens of cats in previous articles (see the article link at the end of the article for details). Gangzhu Medical has also launched a cat…

Animal Viruses

Effectiveness Analysis of Portable Real-Time Fluorescent PCR Instrument for Detecting Animal Viruses

Introduction Animal viruses pose a significant threat to animal health and livestock production. Their spread and prevalence in the farming and livestock industries have a profound impact on human productivity, livelihoods, and public health. To ensure the safety of animal health and livestock production, the detection of animal viruses has become an essential task. Traditional…

Feline Infectious Peritonitis Virus RTPCR Detection

Feline Infectious Peritonitis Virus RTPCR Detection

Introduction Feline infectious peritonitis virus (FIPV) is a pathogen that causes feline infectious peritonitis (FIP) and septicemia in cats. The virus is widespread globally, and there is currently no specific treatment for it. RT-PCR is a highly sensitive, specific, and effective detection method that has been widely used for detecting FIPV nucleic acids. The detection…

Interpretation of Feline Leukemia Virus (FeLV) Test Results

Interpretation of Feline Leukemia Virus (FeLV) Test Results

Introduction In clinical veterinary practice, dealing with diagnostic reports is routine. Often, veterinarians needto combine multiple tests to make a diagnosis. However, sometimes “conflicting” situations arisewhere two or more test results contradict each other, causing doubt and leading to questions aboutthe accuracy of testing methods. This scenario frequently occurs with Feline Leukemia Virus(FeLV). How should…

amplification curve

Index Curve and CT value meaning for q-PCR

In the previous article, we mentioned that qPCR is actually an exponential replication after recognizing a specific DNA, for details, see the previous article, “Introduction to the principle of qPCR detection: amplification principle”. In this article, we will extend the exponential replication, and we will talk about how the amplification curve presented by qPCR results…

Coronavirus

Overview of Digestive Pathogens in Pets—Coronavirus

In the previous article, I mentioned the four main causes of digestive health issues in pets due to hot weather. While three of these causes can be mitigated through early intervention or daily precautions, pathogen infections occur invisibly, making them harder to prevent. Therefore, I would like to create a series on common digestive pathogens,…

Test for Heartworm

How to Test for Heartworm at Home

What is Heartworm Disease? Heartworm disease is a significant parasitic infection caused by Dirofilaria immitis, a nematode transmitted primarily by mosquitoes. Once inside the host, these parasites migrate to the pulmonary arteries and, in severe cases, to the heart, where they mature and reproduce. This condition is not only prevalent among dogs and cats but can…

What Is SDMA

What Is SDMA

Evaluative dimethylarginine (SDMA) is a renal biomarker used to assess glomerular filtration rate for diagnosis, classification, and monitoring of chronic kidney disease. SDMA can diagnose and monitor diseases earlier than traditional renal biomarker testing capabilities. Traditional renal diagnostics 1. Hematology Test Routine blood test, as the most commonly used clinical examination item, has a very…

Canine Babesiosis

Feeling Unwell? Loss of Appetite? An Overview of Prevention and Diagnosis of Canine Babesiosis

What is Babesia? Babesia, commonly referred to as “Babesia microti” , is a type of blood protozoan disease primarily transmitted by ticks. Canine Babesiosis is a global disease, with at least nine distinct genetic types of Babesia reported to infect dogs. Are You Aware of the Risks Babesia Poses to Dogs? The pathogenicity of Babesia…

How to Prevent Cross-Contamination During PCR Test

How to Prevent Cross-Contamination During PCR Test

To prevent cross-contamination in nucleic acid testing, strict adherence to proper laboratory techniques is essential. Here are some key measures: 1. **Physical Separation**: If possible, try not to put the nucleic acid extractor and PCR on the same table. Also reagents and instruments in distance. Trash cans for collecting waste need to have lids. The entire…

Common Methods and Research on Diagnosis of FIP

Common Methods and Research on Diagnosis of FIP

Feline infectious peritonitis (FIP) is a fatal disease. Its pathogen is feline coronavirus (FCoV). This virus is relatively special. FCoV can survive in the intestinal tract of healthy cats. If it is a general disease, it will cause general gastrointestinal symptoms, such as diarrhea. At this time, we call it “feline enteric coronavirus” (FECV). If it…

How to Diagnose Feline Infectious Peritonitis | Common Methods and Research

How to Diagnose Feline Infectious Peritonitis | Common Methods and Research

Feline infectious peritonitis (FIP) is a lethal disease, the pathogen is feline coronavirus (FCoV), this virus is relatively special, FCoV can survive in the intestinal tract of healthy cats, if the general onset of the disease, it will cause general gastrointestinal symptoms, such as diarrhea, at this time, we call the cat coronavirus for feline…

Development Trend of Molecular Diagnostic Technology

Development Trend of Molecular Diagnostic Technology

Countries around the world attach great importance to the development of molecular diagnostic technology, and gene chips will become the mainstream of the development of new-generation molecular diagnostic reagents. Gene chip is the crystallization of the combination of molecular biology, microelectronics, computer and other disciplines. The gene chip has the function of being able to…

Basic process of molecular diagnosis

Basic process of molecular diagnosis

The core technologies of most molecular diagnostic laboratories are focused on detecting specific, relatively short fragments of DNA or RNA. The technology can diagnose infectious diseases, identify specific genetic variants that affect drug metabolism, or detect genes associated with diseases, such as those associated with cancer. At the heart of these assays is the use…

Fundamentals of Molecular Diagnostics

Fundamentals of Molecular Diagnostics

The main technologies of molecular diagnosis are nucleic acid molecular hybridization, polymerase chain reaction and biochip technology. 1. Nucleic acid molecule hybridization technology has a certain complementary sequence and a process in which a nucleotide single strand associates into a heteroduplex in liquid or solid phase according to the principle of base complementary pairing, which…

Introduction to Molecular Diagnostic Technology

Introduction to Molecular Diagnostic Technology

Molecular diagnosis refers to the application of molecular biology methods to detect changes in the structure or expression level of genetic material in patients to make a diagnosis. Molecular diagnosis is the main method for predictive diagnosis, which can be used for the diagnosis of individual genetic diseases and prenatal diagnosis. Molecular diagnosis mainly refers…

What is pet pcr test

What is pet pcr test

The full name of pet PCR detection is viral nucleic acid detection, which is to extract the nucleic acid from pathogenic bacteria or viruses, and through continuous replication, the pathogenic nucleic acid is changed from less to more. What is pet PCR detection At the same time, the technology relies on the enzymatic synthesis reaction…

Common drugs and medical devices

Common drugs and medical devices

Home health equipment: pain massage equipment, home health self-testing equipment, sphygmomanometer, electronic thermometer, multi-function therapeutic equipment, laser therapeutic equipment, blood glucose meter, diabetes therapeutic equipment, vision improvement equipment, sleep improvement equipment, oral hygiene products, Home emergency treatment products. Home health massage products: electric massage chairs/beds, massage sticks, massage hammers, massage pillows, massage cushions, massage belts,…

History of medical devices

History of medical devices

The medical device industry involves many industries such as medicine, machinery, electronics, and plastics. It is a multidisciplinary, knowledge-intensive, and capital-intensive high-tech industry. The basic characteristics of high-tech medical equipment are digitization and computerization. It is the crystallization of multi-disciplinary and cross-domain modern high-tech. Its products have high technical content and high profits. The barriers…

Introduction to Medical Devices

Introduction to Medical Devices

Medical devices refer to instruments, equipment, appliances, in vitro diagnostic reagents and calibrators, materials, and other similar or related items that are directly or indirectly used in the human body, including required computer software. Medical devices include medical equipment and medical consumables. The utility is mainly obtained by physical means, not by pharmacological, immunological or…